Some examples of images generated using various instruments at the Nikon Imaging Center at Northwestern University.
Cytoskeletal UniverseCombined actin, vimentin and microtubule stains in a BHK cell (Rex Chisholm's Lab)
Devil's Angry EyesThis iconic picture of HeLa cells served as the promotional image for the 2011-12 One-Book-One-Northwestern event in which Rebecca Skloot's "The Immortal Life of Henrietta Lacks" was featured. (Teng-Leong Chew's Lab)
Is it a rose?Beta4 integrin (blue) and BPAG1e (magenta) within hemisdesmosomes that have been arranged by keratinocyte into a rose-like pattern (Jonathan Jones' Lab)
Prostate BiopsySpectrally unmixed Ki67-positive nuclei (red) within a highly autofluorescent prostate biopsy specimen. (Peter Gann's lab)
Myosin heads along stress fibersComparison of myosin II sarcomeric periodicity (visualized by GFP-regulatory light chain) along stress fibers, as visualized by conventional confocal microscope (left) and N-SIM structured illumination super-resolution microscope (right). Under N-SIM, the individual globular heads of myosin II are clearly visible, as indicated by the red arrows. (Teng-Leong Chew's Lab)
Ratiometric image of myosin II phosphorylationPtk2 cells were stained with antibody specific for phosphorylated form of myosin II regulatory light chain (RLC-p). The intensity of RLC-p stain was ratioed against GFP-RLC expressed in the cells to create this phosphorylation "heat map". (Rex Chisholm Lab)
Spiky!Espin (green) bundles actin filaments (red) into microvilli that protrude upward from the monolayer of cells. (James Bartles' Lab)